Detailed information of TA system TA175787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4784040..4784590
• Replicon: chromosome
• Accession: NZ_CP062725
• Organism: Escherichia coli O157:H7 strain Z1813

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: ILQ52_RS24150
• Protein ID: WP_001160975.1
• Coordinates: 4784040..4784354 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: ILQ52_RS24155
• Protein ID: WP_000125566.1
• Coordinates: 4784357..4784590 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ52_RS24125	4779715..4781001	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
ILQ52_RS24130	4781001..4781990	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
ILQ52_RS24135	4781987..4782808	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
ILQ52_RS24140	4782811..4783188	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
ILQ52_RS24145	4783195..4784043	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
ILQ52_RS24150	4784040..4784354	-	315	WP_001160975.1	CcdB family protein	Toxin
ILQ52_RS24155	4784357..4784590	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
ILQ52_RS24160	4784676..4785155	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
ILQ52_RS24165	4785347..4787209	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
ILQ52_RS24170	4787202..4787732	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
ILQ52_RS24175	4787840..4789171	-	1332	WP_001183177.1	MFS transporter	-
ILQ52_RS24180	4789229..4789516	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T175787 WP_001160975.1 NZ_CP062725:c4784354-4784040 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T175787 NZ_CP082203:694978-695247 [Streptococcus suis]
ATGGCTTATAAACTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAGTTTGGCAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTGATTTGCGATATTATTGATAACAAAATGGTTATCCTAGCTCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT175787 WP_000125566.1 NZ_CP062725:c4784590-4784357 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT175787 NZ_CP082203:694761-694988 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGGTTAAACGATGCAGAAGAAGAAATCTTCAGAAGCTATGCCATTCATACAGGAAAAAGCCT
GTCTGAGTTATTTAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9