Detailed information of TA system TA175741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2854413..2855051
• Replicon: chromosome
• Accession: NZ_CP062723
• Organism: Escherichia coli O157:H7 strain Z1814

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ILQ53_RS14495
• Protein ID: WP_000813794.1
• Coordinates: 2854875..2855051 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ILQ53_RS14490
• Protein ID: WP_001270286.1
• Coordinates: 2854413..2854829 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ53_RS14470	2849565..2850506	-	942	WP_001251315.1	ABC transporter permease	-
ILQ53_RS14475	2850507..2851520	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
ILQ53_RS14480	2851538..2852683	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
ILQ53_RS14485	2852928..2854334	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
ILQ53_RS14490	2854413..2854829	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ILQ53_RS14495	2854875..2855051	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ILQ53_RS14500	2855273..2855503	+	231	WP_000494244.1	YncJ family protein	-
ILQ53_RS14505	2855595..2857556	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ILQ53_RS14510	2857629..2858165	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
ILQ53_RS14515	2858218..2859429	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T175741 WP_000813794.1 NZ_CP062723:c2855051-2854875 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T175741 NZ_CP082164:c6042586-6042320 [Bradyrhizobium sp. 186]
ATGGCAAACGTTTCTTACACGGAGCTACGGAGCAACCTCGCGGCCTACATGGACCAGGTCTGCGACGATCGCGCCCCGCT
CGTTGTAACGCGCCAGAACGCCCGTAGCGTGGTGATGCTCTCGGAGGAAGAGTACGAGGGCCTTGTGGAGACGGTTCATC
TTCTGAAGAGCCCGGCCAACGCCGCGCGCCTTCTGCGCTCCATCAAGGAAGCCGATGAAGGTAGGCTCGTCGAGCATGAA
TTGATTGAGCCCGGCTCGACTGGCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT175741 WP_001270286.1 NZ_CP062723:c2854829-2854413 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT175741 NZ_CP082164:c6042314-6042048 [Bradyrhizobium sp. 186]
ATGTTGATCGCTTGGACCGAGATTGCCTGGCAAGACTACCTTCAACGGCAAAAAGAGGACGAAAAAATCCTCACGGCCAT
CAACGATTTGATCAAGGACATCAAAAGAGACCCGTTTAGGGGGATCGGGAAACCGGAGCCCCTTAAACATGATTTGCGCG
GTTGGTGGTCTCGCCGCGTTACGGGCGAACATCGGCTGGTGTACCGCCTTCAGGGCAAGGATGACGGGCAGCAGCTCGTC
ATCGCCCAATGTCGATATCACTACTGA

Structures

Toxin

• PDB: 6HPB