Detailed information of TA system TA175718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5002594..5003189
• Replicon: chromosome
• Accession: NZ_CP062721
• Organism: Escherichia coli O157:H7 strain Z1815

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ54_RS24995
• Protein ID: WP_000239579.1
• Coordinates: 5002594..5002944 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ54_RS25000
• Protein ID: WP_001223210.1
• Coordinates: 5002938..5003189 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ54_RS24975	4998040..4999062	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ54_RS24980	4999076..5000578	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
ILQ54_RS24985	5000718..5001674	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ54_RS24990	5001984..5002514	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ54_RS24995	5002594..5002944	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ54_RS25000	5002938..5003189	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ54_RS25005	5003401..5003742	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ54_RS25010	5003745..5007524	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T175718 WP_000239579.1 NZ_CP062721:c5002944-5002594 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T175718 NZ_CP082151:c3564-3226 [Enterobacter cloacae complex sp.]
GTGACGACATATTGTCCAGCCAGAGGCGATGTCATTCTTTTAGATTTCAACCCTCAATCAGGGCATGAGCAGGCCGGGAA
AAGGCCTGCTCTCGTTGTTTCTGATGATTTGTTTAATCAGGTCACTGGGTTTGCTGTTGTTTGTCCTATAACCAATCAAC
AAAAAGGATATCCGTTTGAGGTTTCTGTTGATGGTGCAAAGAAAACGACAGGGGTTATTTTGGCTGATCAAGTGAAGTCT
TTAGACTGGAAGGCAAGAGCTGCCCGAACAGTTGATAGTGTTTCCGATGAGACTGTAACTGCGGTTGTCGGTATGGTTTC
AAAAATTATTAAGGCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT175718 WP_001223210.1 NZ_CP062721:c5003189-5002938 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT175718 NZ_CP082151:c3791-3561 [Enterobacter cloacae complex sp.]
ATGACTACGGCTATCGGTAAATGGGGTAATGGCGCTGCTTTGCGTATCCCGCAGCCGTTCATGAAGCAGCTTGGGCTCAG
TATTGGTGATGCTGTTAATTTCTGCGTCACCGAAAGAAATTTAGTAATCACTAAAGCTGGCCCGACGCTTGAAGAACTGC
TCGGACAGTGCACTGCAGAAAATCGCCATCACGAATATTTTTGTGACTCTCAAGGTAAGGAGATGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8