Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51300..51825 Replicon plasmid pZ1816-1
Accession NZ_CP062720
Organism Escherichia coli O157:H7 strain Z1816

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ55_RS27870 Protein ID WP_001159868.1
Coordinates 51520..51825 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ55_RS27865 Protein ID WP_000813634.1
Coordinates 51300..51518 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ55_RS27835 46583..46813 + 231 WP_010891288.1 hypothetical protein -
ILQ55_RS27840 46934..47674 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ55_RS27845 47959..48936 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ55_RS27850 49344..49544 - 201 WP_000708307.1 hypothetical protein -
ILQ55_RS27855 49541..50161 - 621 WP_001248529.1 hypothetical protein -
ILQ55_RS27860 50158..50841 - 684 WP_010891291.1 DNA-binding protein -
ILQ55_RS27865 51300..51518 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ55_RS27870 51520..51825 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ55_RS27875 51826..52632 + 807 WP_000016989.1 site-specific integrase -
ILQ55_RS27885 54530..54868 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
ILQ55_RS27890 55456..56622 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / toxB 1..92734 92734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T175685 WP_001159868.1 NZ_CP062720:51520-51825 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T175685 NZ_CP082129:1928892-1929146 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT175685 WP_000813634.1 NZ_CP062720:51300-51518 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT175685 NZ_CP082129:1928644-1928895 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References