Detailed information of TA system TA175679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4750863..4751413
• Replicon: chromosome
• Accession: NZ_CP062719
• Organism: Escherichia coli O157:H7 strain Z1816

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: ILQ55_RS23920
• Protein ID: WP_001160975.1
• Coordinates: 4750863..4751177 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: ILQ55_RS23925
• Protein ID: WP_000125566.1
• Coordinates: 4751180..4751413 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ55_RS23895	4746538..4747824	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
ILQ55_RS23900	4747824..4748813	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
ILQ55_RS23905	4748810..4749631	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
ILQ55_RS23910	4749634..4750011	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
ILQ55_RS23915	4750018..4750866	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
ILQ55_RS23920	4750863..4751177	-	315	WP_001160975.1	CcdB family protein	Toxin
ILQ55_RS23925	4751180..4751413	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
ILQ55_RS23930	4751499..4751978	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
ILQ55_RS23935	4752170..4754032	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
ILQ55_RS23940	4754025..4754555	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
ILQ55_RS23945	4754663..4755994	-	1332	WP_001183177.1	MFS transporter	-
ILQ55_RS23950	4756052..4756339	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T175679 WP_001160975.1 NZ_CP062719:c4751177-4750863 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T175679 NZ_CP082129:694081-694395 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT175679 WP_000125566.1 NZ_CP062719:c4751413-4751180 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT175679 NZ_CP082129:694392-694808 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9