Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2803943..2804581 Replicon chromosome
Accession NZ_CP062719
Organism Escherichia coli O157:H7 strain Z1816

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ILQ55_RS14120 Protein ID WP_000813794.1
Coordinates 2803943..2804119 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ILQ55_RS14125 Protein ID WP_001270286.1
Coordinates 2804165..2804581 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ55_RS14100 2799565..2800776 - 1212 WP_071525382.1 BenE family transporter YdcO -
ILQ55_RS14105 2800829..2801365 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ILQ55_RS14110 2801438..2803399 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ILQ55_RS14115 2803491..2803721 - 231 WP_000494244.1 YncJ family protein -
ILQ55_RS14120 2803943..2804119 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ILQ55_RS14125 2804165..2804581 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ILQ55_RS14130 2804660..2806066 + 1407 WP_206338754.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
ILQ55_RS14135 2806311..2807456 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ILQ55_RS14140 2807474..2808487 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ILQ55_RS14145 2808488..2809429 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T175668 WP_000813794.1 NZ_CP062719:2803943-2804119 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T175668 NZ_CP082127:3834339-3834617 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT175668 WP_001270286.1 NZ_CP062719:2804165-2804581 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT175668 NZ_CP082127:3834076-3834336 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References