Detailed information of TA system TA175633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2891284..2891922
• Replicon: chromosome
• Accession: NZ_CP062717
• Organism: Escherichia coli O157:H7 strain Z1825

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ILQ56_RS14775
• Protein ID: WP_000813794.1
• Coordinates: 2891746..2891922 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ILQ56_RS14770
• Protein ID: WP_001270286.1
• Coordinates: 2891284..2891700 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ56_RS14750	2886436..2887377	-	942	WP_001251315.1	ABC transporter permease	-
ILQ56_RS14755	2887378..2888391	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
ILQ56_RS14760	2888409..2889554	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
ILQ56_RS14765	2889799..2891205	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
ILQ56_RS14770	2891284..2891700	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ILQ56_RS14775	2891746..2891922	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ILQ56_RS14780	2892144..2892374	+	231	WP_000494244.1	YncJ family protein	-
ILQ56_RS14785	2892466..2894427	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ILQ56_RS14790	2894500..2895036	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
ILQ56_RS14795	2895089..2896300	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T175633 WP_000813794.1 NZ_CP062717:c2891922-2891746 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T175633 NZ_CP082120:c1869534-1869160 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT175633 WP_001270286.1 NZ_CP062717:c2891700-2891284 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT175633 NZ_CP082120:c1869991-1869623 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 6HPB