Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4219750..4220368 Replicon chromosome
Accession NZ_CP062715
Organism Escherichia coli O157:H7 strain Z1826

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ57_RS21350 Protein ID WP_001291435.1
Coordinates 4220150..4220368 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ57_RS21345 Protein ID WP_000344800.1
Coordinates 4219750..4220124 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ57_RS21335 4214839..4216032 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ57_RS21340 4216055..4219204 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ57_RS21345 4219750..4220124 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ57_RS21350 4220150..4220368 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ57_RS21355 4220540..4221091 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ57_RS21360 4221207..4221677 + 471 WP_000136192.1 YlaC family protein -
ILQ57_RS21365 4221841..4223391 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ57_RS21370 4223433..4223786 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ57_RS21380 4224165..4224476 + 312 WP_000409911.1 MGMT family protein -
ILQ57_RS21385 4224509..4225081 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T175604 WP_001291435.1 NZ_CP062715:4220150-4220368 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T175604 NZ_CP082037:4999765-5000052 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT175604 WP_000344800.1 NZ_CP062715:4219750-4220124 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT175604 NZ_CP082037:4999526-4999765 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References