Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2854717..2855355 Replicon chromosome
Accession NZ_CP062713
Organism Escherichia coli O157:H7 strain Z1830

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ILQ58_RS14510 Protein ID WP_000813794.1
Coordinates 2855179..2855355 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ILQ58_RS14505 Protein ID WP_001270286.1
Coordinates 2854717..2855133 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ58_RS14485 2849869..2850810 - 942 WP_001251315.1 ABC transporter permease -
ILQ58_RS14490 2850811..2851824 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ILQ58_RS14495 2851842..2852987 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ILQ58_RS14500 2853232..2854638 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ILQ58_RS14505 2854717..2855133 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ILQ58_RS14510 2855179..2855355 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ILQ58_RS14515 2855577..2855807 + 231 WP_000494244.1 YncJ family protein -
ILQ58_RS14520 2855899..2857860 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ILQ58_RS14525 2857933..2858469 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ILQ58_RS14530 2858522..2859733 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T175561 WP_000813794.1 NZ_CP062713:c2855355-2855179 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T175561 NZ_CP082029:c735547-735260 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT175561 WP_001270286.1 NZ_CP062713:c2855133-2854717 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT175561 NZ_CP082029:c735786-735547 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References