Detailed information of TA system TA175530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3594257..3594735
• Replicon: chromosome
• Accession: NZ_CP062711
• Organism: Escherichia coli O157:H7 strain Z1831

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ILQ59_RS18495
• Protein ID: WP_001303876.1
• Coordinates: 3594448..3594735 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ILQ59_RS18490
• Protein ID: WP_000536233.1
• Coordinates: 3594257..3594448 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ59_RS18455	3589661..3590431	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
ILQ59_RS18460	3590453..3591199	-	747	WP_000788751.1	ATP-binding protein	-
ILQ59_RS18465	3591206..3592297	-	1092	WP_001205823.1	hypothetical protein	-
ILQ59_RS18470	3592376..3592831	+	456	WP_000273724.1	hypothetical protein	-
ILQ59_RS18475	3593038..3593463	-	426	WP_000693855.1	Rha family transcriptional regulator	-
ILQ59_RS18480	3593447..3593719	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
ILQ59_RS18485	3593828..3594229	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ILQ59_RS18490	3594257..3594448	+	192	WP_000536233.1	hypothetical protein	Antitoxin
ILQ59_RS18495	3594448..3594735	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ILQ59_RS18500	3595013..3595168	+	156	WP_000379575.1	DUF1391 family protein	-
ILQ59_RS18505	3595310..3595699	+	390	WP_000394511.1	hypothetical protein	-
ILQ59_RS18510	3595886..3596071	-	186	WP_001133046.1	hypothetical protein	-
ILQ59_RS18515	3596645..3596833	+	189	WP_000413705.1	cell division inhibition protein DicB	-
ILQ59_RS18520	3596830..3597021	+	192	WP_001098307.1	DUF1482 family protein	-
ILQ59_RS18525	3597115..3599586	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T175530 WP_001303876.1 NZ_CP062711:3594448-3594735 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T175530 NZ_CP082019:c5143875-5143432 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT175530 WP_000536233.1 NZ_CP062711:3594257-3594448 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT175530 NZ_CP082019:c5144102-5143872 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA