Detailed information of TA system TA175508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 409107..409907
• Replicon: chromosome
• Accession: NZ_CP062711
• Organism: Escherichia coli O157:H7 strain Z1831

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: ILQ59_RS01925
• Protein ID: WP_000342448.1
• Coordinates: 409380..409907 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: ILQ59_RS01920
• Protein ID: WP_001277108.1
• Coordinates: 409107..409373 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ59_RS01900	404765..405433	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
ILQ59_RS01905	405426..406484	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
ILQ59_RS01910	406729..407583	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
ILQ59_RS01915	407854..408957	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
ILQ59_RS01920	409107..409373	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
ILQ59_RS01925	409380..409907	+	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
ILQ59_RS01930	409904..410287	-	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
ILQ59_RS01935	410711..411820	+	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
ILQ59_RS01940	411868..412794	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ILQ59_RS01945	412791..414068	+	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
ILQ59_RS01950	414065..414832	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T175508 WP_000342448.1 NZ_CP062711:409380-409907 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T175508 NZ_CP082014:2358926-2359369 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT175508 WP_001277108.1 NZ_CP062711:409107-409373 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT175508 NZ_CP082014:2358699-2358929 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24