Detailed information of TA system TA175505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 25849..26341
• Replicon: plasmid pZ1832-2
• Accession: NZ_CP062710
• Organism: Escherichia coli O157:H7 strain Z1832

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: ILQ60_RS27950
• Protein ID: WP_000702246.1
• Coordinates: 26081..26341 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: ILQ60_RS27945
• Protein ID: WP_021558914.1
• Coordinates: 25849..26091 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ60_RS27910	21076..21411	-	336	WP_000963832.1	hypothetical protein	-
ILQ60_RS27915	22174..22936	+	763	Protein_20	conjugal transfer protein TraL	-
ILQ60_RS27920	22933..23638	+	706	Protein_21	hypothetical protein	-
ILQ60_RS27925	23667..24104	-	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
ILQ60_RS27930	24129..24311	-	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
ILQ60_RS27935	24743..25021	-	279	WP_000545934.1	hypothetical protein	-
ILQ60_RS27940	25079..25408	-	330	WP_021558913.1	hypothetical protein	-
ILQ60_RS27945	25849..26091	-	243	WP_021558914.1	transcriptional regulator	Antitoxin
ILQ60_RS27950	26081..26341	-	261	WP_000702246.1	hypothetical protein	Toxin
ILQ60_RS27955	26393..26617	-	225	WP_000814384.1	hypothetical protein	-
ILQ60_RS27960	26714..26923	-	210	WP_000866037.1	hypothetical protein	-
ILQ60_RS27965	26964..27287	-	324	WP_000432876.1	hypothetical protein	-
ILQ60_RS27970	28993..30006	+	1014	WP_024176825.1	replication initiation protein	-
ILQ60_RS27975	30129..30398	+	270	WP_000190069.1	hypothetical protein	-
ILQ60_RS27980	30680..30928	+	249	WP_072165009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35980	35980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T175505 WP_000702246.1 NZ_CP062710:c26341-26081 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T175505 NZ_CP082014:1713421-1713708 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9258.66 Da
• Isoelectric Point: 9.6823

>AT175505 WP_021558914.1 NZ_CP062710:c26091-25849 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT175505 NZ_CP082014:1713182-1713421 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7