Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 23667..24311 Replicon plasmid pZ1832-2
Accession NZ_CP062710
Organism Escherichia coli O157:H7 strain Z1832

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag ILQ60_RS27930 Protein ID WP_000833471.1
Coordinates 24129..24311 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag ILQ60_RS27925 Protein ID WP_000466317.1
Coordinates 23667..24104 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ60_RS27910 21076..21411 - 336 WP_000963832.1 hypothetical protein -
ILQ60_RS27915 22174..22936 + 763 Protein_20 conjugal transfer protein TraL -
ILQ60_RS27920 22933..23638 + 706 Protein_21 hypothetical protein -
ILQ60_RS27925 23667..24104 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ60_RS27930 24129..24311 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ60_RS27935 24743..25021 - 279 WP_000545934.1 hypothetical protein -
ILQ60_RS27940 25079..25408 - 330 WP_021558913.1 hypothetical protein -
ILQ60_RS27945 25849..26091 - 243 WP_021558914.1 transcriptional regulator -
ILQ60_RS27950 26081..26341 - 261 WP_000702246.1 hypothetical protein -
ILQ60_RS27955 26393..26617 - 225 WP_000814384.1 hypothetical protein -
ILQ60_RS27960 26714..26923 - 210 WP_000866037.1 hypothetical protein -
ILQ60_RS27965 26964..27287 - 324 WP_000432876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35980 35980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T175504 WP_000833471.1 NZ_CP062710:c24311-24129 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T175504 NZ_CP082014:c1577569-1577285 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT175504 WP_000466317.1 NZ_CP062710:c24104-23667 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT175504 NZ_CP082014:c1577801-1577559 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References