Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4217691..4218309 Replicon chromosome
Accession NZ_CP062708
Organism Escherichia coli O157:H7 strain Z1832

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ILQ60_RS21325 Protein ID WP_001291435.1
Coordinates 4218091..4218309 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ILQ60_RS21320 Protein ID WP_000344800.1
Coordinates 4217691..4218065 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ60_RS21310 4212780..4213973 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ILQ60_RS21315 4213996..4217145 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ILQ60_RS21320 4217691..4218065 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ILQ60_RS21325 4218091..4218309 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ILQ60_RS21330 4218481..4219032 + 552 WP_000102553.1 maltose O-acetyltransferase -
ILQ60_RS21335 4219148..4219618 + 471 WP_000136192.1 YlaC family protein -
ILQ60_RS21340 4219782..4221332 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ILQ60_RS21345 4221374..4221727 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ILQ60_RS21355 4222106..4222417 + 312 WP_000409911.1 MGMT family protein -
ILQ60_RS21360 4222450..4223022 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T175494 WP_001291435.1 NZ_CP062708:4218091-4218309 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T175494 NZ_CP082010:c4923266-4922982 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT175494 WP_000344800.1 NZ_CP062708:4217691-4218065 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT175494 NZ_CP082010:c4923498-4923256 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References