Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 366677..367308 Replicon chromosome
Accession NZ_CP062708
Organism Escherichia coli O157:H7 strain Z1832

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag ILQ60_RS01675 Protein ID WP_001259386.1
Coordinates 366677..366952 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ILQ60_RS01680 Protein ID WP_000593555.1
Coordinates 366949..367308 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ60_RS01660 361681..362748 + 1068 WP_000361458.1 HlyD family secretion protein -
ILQ60_RS01665 362745..365480 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ILQ60_RS01670 365480..366604 + 1125 WP_001301659.1 ABC transporter permease -
ILQ60_RS01675 366677..366952 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ60_RS01680 366949..367308 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ60_RS01685 367358..368218 - 861 WP_001131788.1 class II aldolase -
ILQ60_RS01690 368250..368519 - 270 WP_000933060.1 HPr family phosphocarrier protein -
ILQ60_RS01695 368509..370017 - 1509 WP_001301773.1 carbohydrate kinase -
ILQ60_RS01700 370010..371368 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ILQ60_RS01705 371445..371726 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ILQ60_RS01710 371723..372196 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 357821..367308 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T175469 WP_001259386.1 NZ_CP062708:366677-366952 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T175469 NZ_CP082004:c6169-5864 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT175469 WP_000593555.1 NZ_CP062708:366949-367308 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT175469 NZ_CP082004:c6389-6171 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References