Detailed information of TA system TA175467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 25860..26352
• Replicon: plasmid pZ1833-2
• Accession: NZ_CP062707
• Organism: Escherichia coli O157:H7 strain Z1833

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: ILQ61_RS27960
• Protein ID: WP_000702246.1
• Coordinates: 26092..26352 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: ILQ61_RS27955
• Protein ID: WP_021558914.1
• Coordinates: 25860..26102 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ61_RS27920	21080..21417	-	338	Protein_20	plasmid mobilization relaxosome protein MobC	-
ILQ61_RS27925	21765..22084	+	320	Protein_21	hypothetical protein	-
ILQ61_RS27930	22184..22945	+	762	WP_001097938.1	conjugal transfer protein TraL	-
ILQ61_RS27935	22942..23648	+	707	Protein_23	hypothetical protein	-
ILQ61_RS27940	23678..24115	-	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
ILQ61_RS27945	24140..24322	-	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
ILQ61_RS27950	24755..25032	-	278	Protein_26	cobalamin biosynthesis protein CbiX	-
ILQ61_RS27955	25860..26102	-	243	WP_021558914.1	transcriptional regulator	Antitoxin
ILQ61_RS27960	26092..26352	-	261	WP_000702246.1	hypothetical protein	Toxin
ILQ61_RS27965	26726..26935	-	210	WP_000866037.1	hypothetical protein	-
ILQ61_RS27970	26976..27299	-	324	WP_000432876.1	hypothetical protein	-
ILQ61_RS27975	29005..30016	+	1012	Protein_31	replication initiation protein	-
ILQ61_RS27980	30139..30408	+	270	WP_000190069.1	hypothetical protein	-
ILQ61_RS27985	30690..30938	+	249	WP_072165009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35988	35988

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T175467 WP_000702246.1 NZ_CP062707:c26352-26092 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T175467 NZ_CP082002:c5670-5356 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9258.66 Da
• Isoelectric Point: 9.6823

>AT175467 WP_021558914.1 NZ_CP062707:c26102-25860 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT175467 NZ_CP082002:c5936-5658 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7