Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 23678..24322 Replicon plasmid pZ1833-2
Accession NZ_CP062707
Organism Escherichia coli O157:H7 strain Z1833

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag ILQ61_RS27945 Protein ID WP_000833471.1
Coordinates 24140..24322 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag ILQ61_RS27940 Protein ID WP_000466317.1
Coordinates 23678..24115 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ61_RS27915 19597..21083 - 1487 Protein_19 relaxase/mobilization nuclease domain-containing protein -
ILQ61_RS27920 21080..21417 - 338 Protein_20 plasmid mobilization relaxosome protein MobC -
ILQ61_RS27925 21765..22084 + 320 Protein_21 hypothetical protein -
ILQ61_RS27930 22184..22945 + 762 WP_001097938.1 conjugal transfer protein TraL -
ILQ61_RS27935 22942..23648 + 707 Protein_23 hypothetical protein -
ILQ61_RS27940 23678..24115 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ILQ61_RS27945 24140..24322 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
ILQ61_RS27950 24755..25032 - 278 Protein_26 cobalamin biosynthesis protein CbiX -
ILQ61_RS27955 25860..26102 - 243 WP_021558914.1 transcriptional regulator -
ILQ61_RS27960 26092..26352 - 261 WP_000702246.1 hypothetical protein -
ILQ61_RS27965 26726..26935 - 210 WP_000866037.1 hypothetical protein -
ILQ61_RS27970 26976..27299 - 324 WP_000432876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35988 35988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T175466 WP_000833471.1 NZ_CP062707:c24322-24140 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T175466 NZ_CP082001:c5559475-5559092 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT175466 WP_000466317.1 NZ_CP062707:c24115-23678 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT175466 NZ_CP082001:c5559717-5559475 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References