Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 5142..5635 Replicon plasmid pZ1834-2
Accession NZ_CP062704
Organism Escherichia coli O157:H7 strain Z1834

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag ILQ62_RS27530 Protein ID WP_001230709.1
Coordinates 5142..5396 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag ILQ62_RS27535 Protein ID WP_000151456.1
Coordinates 5393..5635 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ62_RS27495 188..490 - 303 WP_001096511.1 hypothetical protein -
ILQ62_RS27500 580..1239 - 660 WP_000516232.1 peptidyl-arginine deiminase -
ILQ62_RS27505 1792..2115 + 324 WP_000432873.1 hypothetical protein -
ILQ62_RS27510 2469..2813 + 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
ILQ62_RS27515 2800..3150 + 351 WP_000127482.1 addiction module antitoxin -
ILQ62_RS27520 3984..4499 + 516 WP_001025393.1 J domain-containing protein -
ILQ62_RS27525 4603..5145 + 543 WP_001083909.1 hypothetical protein -
ILQ62_RS27530 5142..5396 + 255 WP_001230709.1 hypothetical protein Toxin
ILQ62_RS27535 5393..5635 + 243 WP_000151456.1 hypothetical protein Antitoxin
ILQ62_RS27540 6206..6499 + 294 WP_000673431.1 hypothetical protein -
ILQ62_RS27545 6804..7091 + 288 WP_000606467.1 hypothetical protein -
ILQ62_RS27550 7519..7752 + 234 WP_000093745.1 hypothetical protein -
ILQ62_RS27555 7828..8130 + 303 WP_000379301.1 hypothetical protein -
ILQ62_RS27560 8366..8551 + 186 Protein_13 hypothetical protein -
ILQ62_RS27565 8662..9483 + 822 WP_001234372.1 DUF945 domain-containing protein -
ILQ62_RS27570 9780..10382 - 603 WP_072205450.1 transglycosylase SLT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(6)-Id / aph(3'')-Ib - 1..55197 55197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T175429 WP_001230709.1 NZ_CP062704:5142-5396 [Escherichia coli O157:H7]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T175429 NZ_CP081943:c6460397-6459822 [Pseudomonas sp. MM213]
GTGAGGCACTCCTCTTTTACCGCTGTATATGACGCATGTGTGCTGTACCCCGCGCCCCTGAGAGATTTTTTGATGTGGCT
TGCACTCTCCGGACGTTTTAGAGCGAAATGGACAATGGAGATACACAACGAATGGAAACGCAATTTGCTCAAAAACCGTC
CGGATCTGACCACAGGACAACTGAACCGCACGTCCGAACTGATGGATCAGGCATTACCCGACGCTTGCGTGTCCGGTTAC
GAAGAGCTTATTGAAGGACTGACGCTACCCGACGTCGATGATCGACATGTCCTGGCTGTTGCCATTCACTGTAATGCCAG
CGTCATCGTCACCTTCAACCAGAAAGATTTCCCCTGCACCGCCCTGAGCCCCTTCGGTATTGAGGCTCAACACCCTGACG
AGTTCGTCGATAACCTTTTTGATCTCGATCCAGCGGCAGTGATCGAGGCGGCACAACGGCAGCGCAAGCAACTGAAGCTG
CCGCCGATGGATGTCGATAGTTACCTTGAACTTCTGCTGCGTCAGGGCCTGGTTCAATCGAGCAAGGCATTGACCGAGTA
CCGCGCCATTCTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT175429 WP_000151456.1 NZ_CP062704:5393-5635 [Escherichia coli O157:H7]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT175429 NZ_CP081943:c6460861-6460394 [Pseudomonas sp. MM213]
ATGTCACCCACCCTCCATCCGACGATAAACCTCCCGCTGGAGCGCGAAGTGCAGGCTGCTATTGAGGGTCAGCGCGCATT
GGCGGCCTACCTTGCCACTCAATTCGAAACACAGCGCATCCAGATTTTCGACGACCGGAATGAGGCTCACAGCGTCGAAT
TACCCACTTCGGCTCTTCGTTTGCTAGTCGATATCCTGGAAGCGCTCGCTGCGGGCAATGCTGTCAAAGTAGTCCCGGTT
CACGCTGAACTGACGACGCAGGAAGCGGCTGATCTGCTTAACGTCTCACGCCCGCACCTGATCAAACTGCTCGAATCCGG
CGACTTGTCCTACCACAAAACCGGCAAGCACCGACGTGTGCGCTTTGCCGATTTGATGGACTATAAAAACCGGCGCGATA
CCGCCAGCGAGCAAGCAATGATGCTGCTCGCCGAGCAGGCACAAGCGTTAAGGACAGGATACGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References