Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52622..53147 Replicon plasmid pZ1834-1
Accession NZ_CP062703
Organism Escherichia coli O157:H7 strain Z1834

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ILQ62_RS27265 Protein ID WP_001159868.1
Coordinates 52842..53147 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ILQ62_RS27260 Protein ID WP_000813634.1
Coordinates 52622..52840 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ62_RS27230 47905..48135 + 231 WP_010891288.1 hypothetical protein -
ILQ62_RS27235 48256..48996 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ILQ62_RS27240 49281..50258 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ILQ62_RS27245 50666..50866 - 201 WP_000708307.1 hypothetical protein -
ILQ62_RS27250 50863..51483 - 621 WP_206344889.1 hypothetical protein -
ILQ62_RS27255 51480..52163 - 684 WP_010891291.1 DNA-binding protein -
ILQ62_RS27260 52622..52840 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ILQ62_RS27265 52842..53147 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ILQ62_RS27270 53148..53954 + 807 WP_000016989.1 site-specific integrase -
ILQ62_RS27280 56552..57718 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - katA / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..95141 95141


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T175428 WP_001159868.1 NZ_CP062703:52842-53147 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T175428 NZ_CP081943:c3991583-3991218 [Pseudomonas sp. MM213]
ATGGGTTGGGAAATTGAATACACCGATGAATTTGAAGGCTGGTGGGAGAGTTTGGACGCGAAAGAGCAGTCATCGGTGTC
AGCCAGCGTCGATCTGCTTGGGTTACTCGGCCCGGGCCTGCGTTTCCCACATAGCAGCGACATTAAAGGTTCACGACTGG
GCAACCTCCGAGAGTTGCGGGTCCAGCATGCAGGACGTCCCATTCGCGTGCTTTATGCGTTTGATCCACGCCGATGCGCG
TTACTTTTAATCGGAGGGGATAAAACCGGGCAAAACCGTTGGTATGACGAGCATGTCCCGCTGGCTGAAAAACTGTATGA
CGACCATTTGGAAACATTGCGTAAAGAGGGCCGCAACGATGGCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT175428 WP_000813634.1 NZ_CP062703:52622-52840 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT175428 NZ_CP081943:c3991225-3990920 [Pseudomonas sp. MM213]
ATGGCTAAAAAATTTGCTGAACTTCAAGCCCGTATGACGCCAGAGTCTCGCGCAAACGCAGAGCGGTTATTCCAGCAGCA
CCTGAAAGAAATGCCTCTGCACGAGCTGCGTAAGGCCCAGGAGTTGAGCCAGGAAACACTGGCCAAGATGCTGCATGTCA
ATCAGGCGGCGATTTCCAGGATGGAACGACGTACAGACATGTACATCAGCACGCTGCGCAACTACATACGCGCAATGGGT
GGCGAGCTGGAAATCATCGCGACCTTTCCTGAGGGACAGGTAAAGATCGAAAATTTTGCGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References