Detailed information of TA system TA175425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4896011..4896606
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ILQ62_RS24160
• Protein ID: WP_000239579.1
• Coordinates: 4896011..4896361 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ILQ62_RS24165
• Protein ID: WP_001223210.1
• Coordinates: 4896355..4896606 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ62_RS24140	4891457..4892479	-	1023	WP_001301928.1	ABC transporter permease	-
ILQ62_RS24145	4892493..4893995	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
ILQ62_RS24150	4894135..4895091	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ILQ62_RS24155	4895401..4895931	+	531	WP_000055075.1	inorganic diphosphatase	-
ILQ62_RS24160	4896011..4896361	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ILQ62_RS24165	4896355..4896606	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ILQ62_RS24170	4896818..4897159	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ILQ62_RS24175	4897162..4900941	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T175425 WP_000239579.1 NZ_CP062702:c4896361-4896011 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T175425 NZ_CP081942:2129701-2130120 [Pseudomonas sp. MM211]
ATGATCCTGATAGATACCAACGTGCTGTCGGAGCTGATGAAAGCGAAGCCAGCGCTAGAGGTTCTGTCCTGGATTGATCA
GCAGCCTGCAGGTGAGCTTTATATAAGCTCCATTACTGTGGCTGAAATCCTCTATGGAATTGCTCGACTGCCTGACGGTA
AGCGTAAGACGGCTTTTGCTGATCTGGCGGAGTTGATGTTCGATGAGGACTTCTCTGGGCGTGTTCTGCCGTTCGATACA
GATGCAGCGATTCGCTATGCCAGTTTGGCTGCAGCTAGCGAGGCGAAGGGGCGAGTGTCAGATATGGCTGATGCGCAGAT
CGCTGCCATTGCGGCTTTATACGATGCGCCTGTTGCTACGCGTAATGTCCGGCACTTCGATCACCTAGGGGTTCGCGTCA
TAAACCCTTGGGTTGATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT175425 WP_001223210.1 NZ_CP062702:c4896606-4896355 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT175425 NZ_CP081942:2129459-2129704 [Pseudomonas sp. MM211]
ATGGCAACTATCACTATTCGAAATCTGGATGATGACCTCAAGGCCATGCTGCGCGTTGTTGCTGCTAGCCACGGGCGTTC
CATGGAGGAAGAGGCGAGATTGATCATCCGCCAAGCGTTGACCAAGCAAGACAAAAAAGGTGGTTTGGGTAGCCGCATTC
ATGCCCGTTTTTCTGCGGTGGGTGGTGCTGACCTCGAAGTGCCTGATCGAATCACCAAAGCCAAGGCTGCGAGCTTCGAC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8