Detailed information of TA system TA175417

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3588514..3589349
• Replicon: chromosome
• Accession: NZ_CP062702
• Organism: Escherichia coli O157:H7 strain Z1834

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ILQ62_RS18280
• Protein ID: WP_032107687.1
• Coordinates: 3588514..3588891 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ILQ62_RS18285
• Protein ID: WP_033805992.1
• Coordinates: 3588981..3589349 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ62_RS18250	3585195..3585899	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
ILQ62_RS18255	3586184..3586402	+	219	WP_001040187.1	translation initiation factor IF-1	-
ILQ62_RS18265	3586893..3587735	-	843	WP_001684189.1	DUF4942 domain-containing protein	-
ILQ62_RS18270	3587841..3588017	-	177	WP_001684188.1	DUF957 domain-containing protein	-
ILQ62_RS18275	3588029..3588517	-	489	WP_001684187.1	hypothetical protein	-
ILQ62_RS18280	3588514..3588891	-	378	WP_032107687.1	type IV toxin-antitoxin system toxin CbtA	Toxin
ILQ62_RS18285	3588981..3589349	-	369	WP_033805992.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ILQ62_RS18290	3589429..3589650	-	222	WP_001684649.1	DUF987 domain-containing protein	-
ILQ62_RS18295	3589719..3590195	-	477	WP_001186745.1	RadC family protein	-
ILQ62_RS18300	3590211..3590690	-	480	WP_001684386.1	antirestriction protein	-
ILQ62_RS18305	3590856..3591029	-	174	WP_001684385.1	hypothetical protein	-
ILQ62_RS18310	3591029..3591847	-	819	WP_033805991.1	DUF945 domain-containing protein	-
ILQ62_RS18315	3591937..3592160	-	224	Protein_3588	DUF905 family protein	-
ILQ62_RS18320	3592258..3592671	-	414	WP_000789535.1	hypothetical protein	-
ILQ62_RS18325	3592941..3593480	-	540	WP_001104020.1	DUF4339 domain-containing protein	-
ILQ62_RS18330	3593605..3594062	-	458	Protein_3591	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13903.98 Da
• Isoelectric Point: 7.7785

>T175417 WP_032107687.1 NZ_CP062702:c3588891-3588514 [Escherichia coli O157:H7]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTCSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGVKQ

Nucleotide

• Length: 378 bp

>T175417 NZ_CP081939:c305364-304945 [Avibacterium paragallinarum]
ATGTATTTATTAGACACGAACATTATTTCCGAACTTTATAAATTTCGTAACGGAAAAATTAATGCGGGTGTAAAAGCGTG
GTTGGCTGAGATTTTACCGAGTGAAACCGCGATCAGTTGCATTACACTTTCAGAAATTCAAACGGGTATTTTGTTAAAGG
CACGTCGGGACAAAACTCAAGCGGCGTTGTTAAATGAATGGTTTAAGCAACAAATCATTCCTGTTTATCAACAAAATACC
TTACCTATTACAGCAGAAATTGCATTAATGGCGGCGGAATTGCACATTCCAAATAAAATGGATATTAATGATGCCTACAT
TGCCGCCACCGCAAAATTTCATCGTCTGAAATTAGTTACCCGCAATATCAAAGATTTTGAAAATTGCGGGATAGAAATTA
TCAATCCATTTCTTGATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13578.33 Da
• Isoelectric Point: 6.7384

>AT175417 WP_033805992.1 NZ_CP062702:c3589349-3588981 [Escherichia coli O157:H7]
VSDTLHETNHPDGNNNSPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGQFSDADVYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK

Nucleotide

• Length: 369 bp

>AT175417 NZ_CP081939:c305655-305380 [Avibacterium paragallinarum]
ATGGCAATTACTGTTACCTCAAAAGAATTTAATCAACGTGCTAGCTATGTATTGAAACTCTCTGAAACCCAGCCTGTTTT
TATTACCAAATGGGGAAAGATTGTCAGTGTGTTGTCGAACTATCGTGATTACCGCAAAACCCAATCAGAACCGAGTTTTG
AGGAGCTATTTGGGCAACCTACACCCCCTGTTAGCGATAAAGACATTGAAGATTTCGATCAAATTCTTACGGAAATTCGC
AAAAATACGCATATACGCGAAGTGGAATTGGATTAG