Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 938815..939469 Replicon chromosome
Accession NZ_CP062702
Organism Escherichia coli O157:H7 strain Z1834

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ILQ62_RS04590 Protein ID WP_000244781.1
Coordinates 939062..939469 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ILQ62_RS04585 Protein ID WP_000354046.1
Coordinates 938815..939081 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ62_RS04565 934903..936336 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
ILQ62_RS04570 936381..936692 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ILQ62_RS04575 936856..937515 + 660 WP_000250274.1 hemolysin III family protein -
ILQ62_RS04580 937592..938572 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
ILQ62_RS04585 938815..939081 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ILQ62_RS04590 939062..939469 + 408 WP_000244781.1 protein YgfX Toxin
ILQ62_RS04595 939509..940030 - 522 WP_001055874.1 flavodoxin FldB -
ILQ62_RS04600 940142..941038 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ILQ62_RS04605 941063..941773 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ILQ62_RS04610 941779..943512 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T175399 WP_000244781.1 NZ_CP062702:939062-939469 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T175399 NZ_CP081896:c4660669-4660385 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT175399 WP_000354046.1 NZ_CP062702:938815-939081 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT175399 NZ_CP081896:c4660901-4660659 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References