Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 403134..403934 Replicon chromosome
Accession NZ_CP062702
Organism Escherichia coli O157:H7 strain Z1834

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag ILQ62_RS01895 Protein ID WP_000342448.1
Coordinates 403407..403934 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag ILQ62_RS01890 Protein ID WP_001277108.1
Coordinates 403134..403400 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILQ62_RS01870 398792..399460 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
ILQ62_RS01875 399453..400511 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
ILQ62_RS01880 400756..401610 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
ILQ62_RS01885 401881..402984 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
ILQ62_RS01890 403134..403400 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
ILQ62_RS01895 403407..403934 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
ILQ62_RS01900 403931..404314 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
ILQ62_RS01905 404738..405847 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
ILQ62_RS01910 405895..406821 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ILQ62_RS01915 406818..408095 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
ILQ62_RS01920 408092..408859 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T175394 WP_000342448.1 NZ_CP062702:403407-403934 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T175394 NZ_CP081896:1821313-1821415 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT175394 WP_001277108.1 NZ_CP062702:403134-403400 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT175394 NZ_CP081896:c1821422-1821277 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References