Detailed information of TA system TA175377

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3276789..3277584
• Replicon: chromosome
• Accession: NZ_CP062700
• Organism: Escherichia coli O157:H7 strain Z1835

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: ILQ63_RS16760
• Protein ID: WP_000854914.1
• Coordinates: 3276789..3277163 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ILQ63_RS16765
• Protein ID: WP_001416641.1
• Coordinates: 3277210..3277584 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILQ63_RS16720	3271793..3272284	-	492	WP_001301587.1	DUF1097 domain-containing protein	-
ILQ63_RS16725	3272386..3272940	-	555	WP_001001917.1	molecular chaperone YcdY	-
ILQ63_RS16730	3272964..3273701	-	738	WP_000283664.1	zinc-binding phosphatase	-
ILQ63_RS16735	3273756..3274694	-	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
ILQ63_RS16745	3275165..3276007	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
ILQ63_RS16750	3276092..3276289	-	198	WP_000839282.1	DUF957 domain-containing protein	-
ILQ63_RS16755	3276301..3276792	-	492	WP_000976853.1	hypothetical protein	-
ILQ63_RS16760	3276789..3277163	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
ILQ63_RS16765	3277210..3277584	-	375	WP_001416641.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ILQ63_RS16770	3277747..3277968	-	222	WP_001220314.1	DUF987 domain-containing protein	-
ILQ63_RS16775	3278031..3278507	-	477	WP_001415969.1	RadC family protein	-
ILQ63_RS16780	3278523..3279008	-	486	WP_000214398.1	antirestriction protein	-
ILQ63_RS16785	3279099..3279917	-	819	WP_001234682.1	DUF945 domain-containing protein	-
ILQ63_RS16790	3280257..3281327	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
ILQ63_RS16795	3281324..3282229	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T175377 WP_000854914.1 NZ_CP062700:c3277163-3276789 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T175377 NZ_CP081894:c2632544-2631222 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13778.61 Da
• Isoelectric Point: 6.6249

>AT175377 WP_001416641.1 NZ_CP062700:c3277584-3277210 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT175377 NZ_CP081894:c2632810-2632544 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5