Detailed information of TA system TA175352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1763690..1765419
• Replicon: chromosome
• Accession: NZ_CP062699
• Organism: Pseudomonas taiwanensis strain WRS8

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: I3UUH3
• Locus tag: ICN73_RS08010
• Protein ID: WP_014754748.1
• Coordinates: 1763690..1764331 (+)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: I3UUH2
• Locus tag: ICN73_RS08015
• Protein ID: WP_014754747.1
• Coordinates: 1764331..1765419 (+)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN73_RS07990	1759392..1759907	+	516	WP_003249048.1	dihydrofolate reductase	-
ICN73_RS07995	1760326..1761717	-	1392	WP_003249050.1	L-cystine transporter	-
ICN73_RS08000	1761962..1763017	+	1056	WP_014754750.1	phosphorylcholine phosphatase	-
ICN73_RS08005	1763115..1763693	+	579	WP_019752696.1	DUF4433 domain-containing protein	-
ICN73_RS08010	1763690..1764331	+	642	WP_014754748.1	DUF4433 domain-containing protein	Toxin
ICN73_RS08015	1764331..1765419	+	1089	WP_014754747.1	macro domain-containing protein	Antitoxin
ICN73_RS08020	1765487..1767328	-	1842	WP_003249056.1	dihydroxy-acid dehydratase	-
ICN73_RS08025	1767599..1768795	-	1197	WP_004575470.1	class I SAM-dependent rRNA methyltransferase	-
ICN73_RS08030	1768971..1769789	+	819	WP_173881454.1	HDOD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24590.18 Da
• Isoelectric Point: 9.5438

>T175352 WP_014754748.1 NZ_CP062699:1763690-1764331 [Pseudomonas taiwanensis]
MIYSSLNPSKALIWRIVHRDNLSWVLDHGLHAVSSGKLSSTYVGIGNPELISKRAQRRVPLEPNGTLADYVPFYFTPFSV
MMKNIHSGWGVPQRRNEEIVILVSSLYHAQSLGLQFVFTDAHAYPHWAGYYSNLTQLDKIDWEILQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAALLGIVCYNEALKGRVDKEVQQRRLNLPVYARPGWYF

Nucleotide

• Length: 642 bp

>T175352 NZ_CP081891:c4759476-4759192 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40625.75 Da
• Isoelectric Point: 6.5916

>AT175352 WP_014754747.1 NZ_CP062699:1764331-1765419 [Pseudomonas taiwanensis]
MIHFMQGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFVENYRQYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVEGLHDLRNFIINNQVASIAIPPLGAGNGGLSWAAVREQIQRVLGDLDADVFVFEPTANYQNVSKRSG
VEVLTPARALIAELVRRYWVLGMECSLLEVQKLAWFLERSIERFTPGINPLELKFVAHKYGPYANRLEHLLDNLDGSYLH
CRKRISDAGPLDVIWFDEGRKSFLQAYLNSEAKAYLPALEFTAELIDGFESPFGMELLATVDWLLHEDGVEPTVPAVREH
LKNWSGGVGAAARKSKLFDDEALGIALQKLSMFRSDPATSLH

Nucleotide

• Length: 1089 bp

>AT175352 NZ_CP081891:c4759708-4759466 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: I3UUH3

Antitoxin

• AlphaFold DB: I3UUH2