Detailed information of TA system TA175351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 1146155..1147023
• Replicon: chromosome
• Accession: NZ_CP062699
• Organism: Pseudomonas taiwanensis strain WRS8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICN73_RS05235
• Protein ID: WP_172402800.1
• Coordinates: 1146670..1147023 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q88R60
• Locus tag: ICN73_RS05230
• Protein ID: WP_004575922.1
• Coordinates: 1146155..1146628 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN73_RS05200	1141773..1142636	-	864	Protein_1006	phage portal protein	-
ICN73_RS05205	1142634..1143428	+	795	Protein_1007	integrase	-
ICN73_RS05210	1143532..1144674	+	1143	WP_014755049.1	DUF4935 domain-containing protein	-
ICN73_RS05215	1145199..1145522	+	324	WP_011953230.1	helix-turn-helix domain-containing protein	-
ICN73_RS05220	1145519..1145734	-	216	WP_011953229.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ICN73_RS05230	1146155..1146628	-	474	WP_004575922.1	transcriptional regulator	Antitoxin
ICN73_RS05235	1146670..1147023	-	354	WP_172402800.1	type II toxin-antitoxin system HigB family toxin	Toxin
ICN73_RS05240	1147205..1147411	+	207	WP_011953227.1	DUF3079 domain-containing protein	-
ICN73_RS05245	1147562..1149859	-	2298	WP_014755047.1	TonB-dependent siderophore receptor	-
ICN73_RS05250	1150000..1150719	+	720	WP_010951640.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13672.88 Da
• Isoelectric Point: 10.6182

>T175351 WP_172402800.1 NZ_CP062699:c1147023-1146670 [Pseudomonas taiwanensis]
MRVITKAAVTKAIEAHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWVTVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 354 bp

>T175351 NZ_CP081891:c4667499-4667158 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACGCCTCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17229.67 Da
• Isoelectric Point: 4.4982

>AT175351 WP_004575922.1 NZ_CP062699:c1146628-1146155 [Pseudomonas taiwanensis]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT175351 NZ_CP081891:c4667861-4667520 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGTTGACCGGGCCAGCATTACCGGTGACTTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CATTTACCCCACTCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CBJ4