Detailed information of TA system TA175350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 493559..494145
• Replicon: chromosome
• Accession: NZ_CP062699
• Organism: Pseudomonas taiwanensis strain WRS8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICN73_RS02285
• Protein ID: WP_012051236.1
• Coordinates: 493852..494145 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: ICN73_RS02280
• Protein ID: WP_004576347.1
• Coordinates: 493559..493855 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN73_RS02255	488936..489430	-	495	WP_014755382.1	RidA family protein	-
ICN73_RS02260	489510..490901	-	1392	WP_017148108.1	FAD-binding oxidoreductase	-
ICN73_RS02265	491486..491908	-	423	WP_012051239.1	VOC family protein	-
ICN73_RS02270	492251..492898	+	648	WP_017148106.1	serine protease	-
ICN73_RS02275	493069..493548	+	480	WP_012051237.1	GNAT family N-acetyltransferase	-
ICN73_RS02280	493559..493855	-	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
ICN73_RS02285	493852..494145	-	294	WP_012051236.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICN73_RS02290	494367..495260	+	894	WP_012051235.1	hypothetical protein	-
ICN73_RS02295	495297..496064	-	768	WP_003248573.1	phosphonate ABC transporter, permease protein PhnE	-
ICN73_RS02300	496061..496888	-	828	WP_003248575.1	ABC transporter permease subunit	-
ICN73_RS02305	496882..497691	-	810	WP_014755377.1	phosphonate ABC transporter ATP-binding protein	-
ICN73_RS02310	497688..498542	-	855	WP_014755376.1	putative selenate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-92)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10648.42 Da
• Isoelectric Point: 10.6919

>T175350 WP_012051236.1 NZ_CP062699:c494145-493852 [Pseudomonas taiwanensis]
MFLVEQTESFAAWLSSLRDLSARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVDFTQKGQRLVLLLVGGDKS
TQAADILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T175350 NZ_CP081891:c4632163-4631630 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT175350 WP_004576347.1 NZ_CP062699:c493855-493559 [Pseudomonas taiwanensis]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT175350 NZ_CP081891:c4632440-4632174 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CD26