Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 510251..510887 Replicon chromosome
Accession NZ_CP062497
Organism Bacillus subtilis strain CV16

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IHV08_RS02610 Protein ID WP_003156187.1
Coordinates 510537..510887 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IHV08_RS02605 Protein ID WP_003225183.1
Coordinates 510251..510532 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHV08_RS02585 506610..507209 - 600 WP_192858216.1 rhomboid family intramembrane serine protease -
IHV08_RS02590 507304..507669 + 366 WP_014475874.1 holo-ACP synthase -
IHV08_RS02595 507835..508851 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
IHV08_RS02600 508966..510135 + 1170 WP_192858217.1 alanine racemase -
IHV08_RS02605 510251..510532 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IHV08_RS02610 510537..510887 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IHV08_RS02615 511002..511826 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
IHV08_RS02620 511831..512196 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
IHV08_RS02625 512200..512601 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
IHV08_RS02630 512613..513620 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
IHV08_RS02635 513682..514011 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
IHV08_RS02640 514008..514490 + 483 WP_003234299.1 anti-sigma B factor RsbW -
IHV08_RS02645 514456..515244 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
IHV08_RS02650 515244..515843 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T175334 WP_003156187.1 NZ_CP062497:510537-510887 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T175334 NZ_CP081890:1835015-1835117 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT175334 WP_003225183.1 NZ_CP062497:510251-510532 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT175334 NZ_CP081890:c1835124-1834979 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References