Detailed information of TA system TA175332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 2535764..2536241
• Replicon: chromosome
• Accession: NZ_CP062471
• Organism: Staphylococcus aureus strain MRSA - AMRF 3

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: ILP75_RS12380
• Protein ID: WP_000052076.1
• Coordinates: 2535764..2535952 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: ILP75_RS12385
• Protein ID: WP_001059690.1
• Coordinates: 2535981..2536241 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILP75_RS12350	2531754..2532275	+	522	WP_000824487.1	hypothetical protein	-
ILP75_RS12355	2532616..2533302	+	687	WP_001793574.1	thermonuclease family protein	-
ILP75_RS12360	2533659..2533859	+	201	WP_001059082.1	cold-shock protein	-
ILP75_RS12365	2534355..2534573	-	219	WP_000005886.1	hypothetical protein	-
ILP75_RS12370	2534635..2534919	-	285	WP_001004399.1	hypothetical protein	-
ILP75_RS12375	2535007..2535576	-	570	WP_001106652.1	hypothetical protein	-
ILP75_RS12380	2535764..2535952	-	189	WP_000052076.1	hypothetical protein	Toxin
ILP75_RS12385	2535981..2536241	-	261	WP_001059690.1	hypothetical protein	Antitoxin
ILP75_RS12390	2536582..2536785	+	204	WP_000145164.1	sterile alpha motif-like domain-containing protein	-
ILP75_RS12395	2536782..2537018	-	237	WP_000368687.1	hypothetical protein	-
ILP75_RS12400	2537243..2537824	+	582	WP_001140695.1	phosphoglycerate mutase family protein	-
ILP75_RS12405	2537897..2538514	-	618	WP_000255557.1	LysE/ArgO family amino acid transporter	-
ILP75_RS12410	2538669..2539163	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
ILP75_RS12415	2539562..2539984	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
ILP75_RS12420	2540132..2540848	+	717	WP_000150016.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	dfrG	sec / sell / vWbp	2499228..2537824	38596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T175332 WP_000052076.1 NZ_CP062471:c2535952-2535764 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T175332 NZ_CP081890:729677-730162 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT175332 WP_001059690.1 NZ_CP062471:c2536241-2535981 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT175332 NZ_CP081890:729387-729680 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56