Detailed information of TA system TA175331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2505292..2505821
• Replicon: chromosome
• Accession: NZ_CP062471
• Organism: Staphylococcus aureus strain MRSA - AMRF 3

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: ILP75_RS12215
• Protein ID: WP_001103939.1
• Coordinates: 2505504..2505821 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: ILP75_RS12210
• Protein ID: WP_001058492.1
• Coordinates: 2505292..2505501 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILP75_RS12175	2501622..2502086	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
ILP75_RS12185	2502649..2503755	-	1107	WP_000149511.1	site-specific integrase	-
ILP75_RS12190	2503745..2504548	-	804	WP_000358990.1	helix-turn-helix domain-containing protein	-
ILP75_RS12195	2504684..2504887	+	204	WP_001045296.1	transcriptional regulator	-
ILP75_RS12200	2504923..2505141	+	219	WP_000163544.1	helix-turn-helix domain-containing protein	-
ILP75_RS12205	2505153..2505299	+	147	WP_000784885.1	hypothetical protein	-
ILP75_RS12210	2505292..2505501	+	210	WP_001058492.1	hypothetical protein	Antitoxin
ILP75_RS12215	2505504..2505821	+	318	WP_001103939.1	DUF1474 family protein	Toxin
ILP75_RS12220	2505885..2506754	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
ILP75_RS12225	2506771..2508228	+	1458	WP_000390453.1	virulence-associated E family protein	-
ILP75_RS12230	2508529..2508891	+	363	WP_001039170.1	hypothetical protein	-
ILP75_RS12235	2508893..2509177	+	285	WP_000998185.1	hypothetical protein	-
ILP75_RS12240	2509174..2509815	+	642	WP_001019807.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	dfrG	sec / sell / vWbp	2499228..2537824	38596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T175331 WP_001103939.1 NZ_CP062471:2505504-2505821 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T175331 NZ_CP081890:350626-350973 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT175331 WP_001058492.1 NZ_CP062471:2505292-2505501 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT175331 NZ_CP081890:350973-351272 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IA75