Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 981166..981695 Replicon chromosome
Accession NZ_CP062471
Organism Staphylococcus aureus strain MRSA - AMRF 3

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag ILP75_RS04880 Protein ID WP_000621175.1
Coordinates 981166..981528 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag ILP75_RS04885 Protein ID WP_000948331.1
Coordinates 981525..981695 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILP75_RS04860 978145..978915 - 771 WP_001041102.1 RNA polymerase sigma factor SigB -
ILP75_RS04865 978890..979369 - 480 WP_001190829.1 anti-sigma B factor RsbW -
ILP75_RS04870 979371..979697 - 327 WP_001052491.1 anti-sigma factor antagonist -
ILP75_RS04875 979816..980817 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
ILP75_RS04880 981166..981528 - 363 WP_000621175.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ILP75_RS04885 981525..981695 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ILP75_RS04890 981780..982928 - 1149 WP_001281154.1 alanine racemase -
ILP75_RS04895 982994..983353 - 360 WP_000581197.1 holo-ACP synthase -
ILP75_RS04900 983357..983848 - 492 WP_001205912.1 PH domain-containing protein -
ILP75_RS04905 983835..985418 - 1584 WP_001294620.1 PH domain-containing protein -
ILP75_RS04910 985411..985890 - 480 WP_001287079.1 hypothetical protein -
ILP75_RS04915 986099..986659 - 561 WP_001092409.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13441.69 Da        Isoelectric Point: 10.1654

>T175323 WP_000621175.1 NZ_CP062471:c981528-981166 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Download         Length: 363 bp

>T175323 NZ_CP081889:1860942-1861044 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT175323 WP_000948331.1 NZ_CP062471:c981695-981525 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT175323 NZ_CP081889:c1861051-1860906 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References