Detailed information of TA system TA175312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 918043..918572
• Replicon: chromosome
• Accession: NZ_CP062469
• Organism: Staphylococcus aureus strain MRSA - AMRF 4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ILP76_RS04395
• Protein ID: WP_000621177.1
• Coordinates: 918210..918572 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ILP76_RS04390
• Protein ID: WP_000948331.1
• Coordinates: 918043..918213 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILP76_RS04360	913079..913639	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
ILP76_RS04365	913848..914327	+	480	WP_001287078.1	hypothetical protein	-
ILP76_RS04370	914320..915903	+	1584	WP_001294627.1	PH domain-containing protein	-
ILP76_RS04375	915890..916381	+	492	WP_001205910.1	PH domain-containing protein	-
ILP76_RS04380	916385..916744	+	360	WP_000581202.1	holo-ACP synthase	-
ILP76_RS04385	916810..917958	+	1149	WP_001281147.1	alanine racemase	-
ILP76_RS04390	918043..918213	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ILP76_RS04395	918210..918572	+	363	WP_000621177.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ILP76_RS04400	918922..919923	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ILP76_RS04405	920042..920368	+	327	WP_001052491.1	anti-sigma factor antagonist	-
ILP76_RS04410	920370..920849	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
ILP76_RS04415	920824..921594	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13471.72 Da
• Isoelectric Point: 10.1654

>T175312 WP_000621177.1 NZ_CP062469:918210-918572 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNTVAHQKN

Nucleotide

• Length: 363 bp

>T175312 NZ_CP081881:c4277537-4277187 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175312 WP_000948331.1 NZ_CP062469:918043-918213 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175312 NZ_CP081881:c4277782-4277531 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1