Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 898300..898814 Replicon chromosome
Accession NZ_CP062469
Organism Staphylococcus aureus strain MRSA - AMRF 4

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag ILP76_RS04305 Protein ID WP_000581792.1
Coordinates 898605..898814 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag ILP76_RS04300 Protein ID WP_000138932.1
Coordinates 898300..898593 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILP76_RS04280 893910..894551 + 642 WP_000483153.1 thiamine phosphate synthase -
ILP76_RS04285 894637..895509 + 873 WP_000725802.1 membrane protein insertase YidC -
ILP76_RS04290 895975..896622 - 648 WP_001187630.1 HD domain-containing protein -
ILP76_RS04295 896650..898134 - 1485 WP_000571549.1 cardiolipin synthase -
ILP76_RS04300 898300..898593 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
ILP76_RS04305 898605..898814 + 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
ILP76_RS04310 898942..899079 + 138 WP_000828354.1 hypothetical protein -
ILP76_RS04315 899343..900545 - 1203 WP_001109936.1 rod shape-determining protein RodA -
ILP76_RS04320 900863..901933 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
ILP76_RS04325 901948..903306 + 1359 WP_000611459.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T175311 WP_000581792.1 NZ_CP062469:898605-898814 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T175311 NZ_CP081881:c4266133-4265843 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT175311 WP_000138932.1 NZ_CP062469:898300-898593 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT175311 NZ_CP081881:c4266365-4266123 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References