Detailed information of TA system TA175289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1611262..1611791
• Replicon: chromosome
• Accession: NZ_CP062467
• Organism: Staphylococcus aureus strain MRSA - AMRF 5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ILP77_RS07920
• Protein ID: WP_000621175.1
• Coordinates: 1611429..1611791 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ILP77_RS07915
• Protein ID: WP_000948331.1
• Coordinates: 1611262..1611432 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILP77_RS07885	1606298..1606858	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
ILP77_RS07890	1607067..1607546	+	480	WP_001287079.1	hypothetical protein	-
ILP77_RS07895	1607539..1609122	+	1584	WP_001294620.1	PH domain-containing protein	-
ILP77_RS07900	1609109..1609600	+	492	WP_001205912.1	PH domain-containing protein	-
ILP77_RS07905	1609604..1609963	+	360	WP_000581197.1	holo-ACP synthase	-
ILP77_RS07910	1610029..1611177	+	1149	WP_001281154.1	alanine racemase	-
ILP77_RS07915	1611262..1611432	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ILP77_RS07920	1611429..1611791	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ILP77_RS07925	1612140..1613141	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ILP77_RS07930	1613260..1613586	+	327	WP_001052491.1	anti-sigma factor antagonist	-
ILP77_RS07935	1613588..1614067	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
ILP77_RS07940	1614042..1614812	+	771	WP_194085749.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175289 WP_000621175.1 NZ_CP062467:1611429-1611791 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175289 NZ_CP081881:c71144-70767 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGATACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAACATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175289 WP_000948331.1 NZ_CP062467:1611262-1611432 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175289 NZ_CP081881:c71565-71191 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCACCTGCATTACCTTGCCGACCGCGCGGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1