Detailed information of TA system TA175270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 870680..871209
• Replicon: chromosome
• Accession: NZ_CP062465
• Organism: Staphylococcus aureus strain MRSA - AMRF 6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ILP78_RS04190
• Protein ID: WP_000621177.1
• Coordinates: 870847..871209 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: ILP78_RS04185
• Protein ID: WP_000948331.1
• Coordinates: 870680..870850 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ILP78_RS04155	865716..866276	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
ILP78_RS04160	866485..866964	+	480	WP_001287078.1	hypothetical protein	-
ILP78_RS04165	866957..868540	+	1584	WP_001294627.1	PH domain-containing protein	-
ILP78_RS04170	868527..869018	+	492	WP_001205910.1	PH domain-containing protein	-
ILP78_RS04175	869022..869381	+	360	WP_000581202.1	holo-ACP synthase	-
ILP78_RS04180	869447..870595	+	1149	WP_001281147.1	alanine racemase	-
ILP78_RS04185	870680..870850	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
ILP78_RS04190	870847..871209	+	363	WP_000621177.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ILP78_RS04195	871559..872560	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
ILP78_RS04200	872679..873005	+	327	WP_001052491.1	anti-sigma factor antagonist	-
ILP78_RS04205	873007..873486	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
ILP78_RS04210	873461..874231	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13471.72 Da
• Isoelectric Point: 10.1654

>T175270 WP_000621177.1 NZ_CP062465:870847-871209 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNTVAHQKN

Nucleotide

• Length: 363 bp

>T175270 NZ_CP081865:357348-357695 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175270 WP_000948331.1 NZ_CP062465:870680-870850 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175270 NZ_CP081865:357695-357994 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1