Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 850937..851451 Replicon chromosome
Accession NZ_CP062465
Organism Staphylococcus aureus strain MRSA - AMRF 6

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag ILP78_RS04100 Protein ID WP_000581792.1
Coordinates 851242..851451 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag ILP78_RS04095 Protein ID WP_000138932.1
Coordinates 850937..851230 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ILP78_RS04075 846547..847188 + 642 WP_000483153.1 thiamine phosphate synthase -
ILP78_RS04080 847274..848146 + 873 WP_000725802.1 membrane protein insertase YidC -
ILP78_RS04085 848612..849259 - 648 WP_001187630.1 HD domain-containing protein -
ILP78_RS04090 849287..850771 - 1485 WP_000571549.1 cardiolipin synthase -
ILP78_RS04095 850937..851230 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
ILP78_RS04100 851242..851451 + 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
ILP78_RS04105 851579..851716 + 138 WP_000828354.1 hypothetical protein -
ILP78_RS04110 851980..853182 - 1203 WP_001109936.1 rod shape-determining protein RodA -
ILP78_RS04115 853500..854570 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
ILP78_RS04120 854585..855943 + 1359 WP_000611459.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T175269 WP_000581792.1 NZ_CP062465:851242-851451 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T175269 NZ_CP081865:331379-331747 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT175269 WP_000138932.1 NZ_CP062465:850937-851230 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT175269 NZ_CP081865:331161-331382 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References