Detailed information of TA system TA175261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2743608..2744137
• Replicon: chromosome
• Accession: NZ_CP062439
• Organism: Staphylococcus aureus strain NAS_AN_285

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF743_RS13670
• Protein ID: WP_000621175.1
• Coordinates: 2743775..2744137 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF743_RS13665
• Protein ID: WP_000948331.1
• Coordinates: 2743608..2743778 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF743_RS13635 (2738645)	2738645..2739205	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF743_RS13640 (2739413)	2739413..2739892	+	480	WP_001287088.1	hypothetical protein	-
IF743_RS13645 (2739885)	2739885..2741468	+	1584	WP_001294626.1	PH domain-containing protein	-
IF743_RS13650 (2741455)	2741455..2741946	+	492	WP_001205910.1	PH domain-containing protein	-
IF743_RS13655 (2741950)	2741950..2742309	+	360	WP_000581200.1	holo-ACP synthase	-
IF743_RS13660 (2742375)	2742375..2743523	+	1149	WP_001281145.1	alanine racemase	-
IF743_RS13665 (2743608)	2743608..2743778	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF743_RS13670 (2743775)	2743775..2744137	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF743_RS13675 (2744486)	2744486..2745487	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF743_RS13680 (2745606)	2745606..2745932	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF743_RS13685 (2745934)	2745934..2746413	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF743_RS13690 (2746388)	2746388..2747158	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175261 WP_000621175.1 NZ_CP062439:2743775-2744137 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175261 NZ_CP081864:c1035885-1035631 [Symbiopectobacterium purcellii]
ATGAAGTCAACGTGGTCTGAAGAAGCATGGGAGGATTATCTGTACTGGCAGGATACAGATAATCGCATGGTTAAGAAGAT
CAACGCACTCATTAAAGATGTTTTGAGAACGCCTTTTGAGGGTAAGGGTAAACCTGAGCCCTTGAAACACAATTTGTCCG
GTTTTTGGTCCCGGCGCATCACCGAAGAGCATCGTCTGGTTTATGCCATTGCAGAGGGTGCCATTCTTATAGCCGCCTGC
CGCTACCACTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175261 WP_000948331.1 NZ_CP062439:2743608-2743778 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175261 NZ_CP081864:c1036133-1035882 [Symbiopectobacterium purcellii]
ATGAGAACGATCAGTTACAGTGAAGCCCGCCAGAATTTATCAGCAACGATGATGAAAACGGTGGAAGATCACGCCCCCAT
ATTGATTACCCGGCAAAACGGTGAGGCGTGTGTTCTCATGTCCCTGGAAGAGTACAACTCTCTGGAAGAAACCGCCTATC
TGTTACGCTCGCCGGCAAATGCCAAAAGGCTTATGAACGCCATAGAAAGTTTGAAAGCCGGTGAAGGGACAGAAAAGGGC
ATCATCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1