Detailed information of TA system TA175224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2649062..2649591
• Replicon: chromosome
• Accession: NZ_CP062420
• Organism: Staphylococcus aureus strain NAS_AN_016

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF696_RS13115
• Protein ID: WP_000621175.1
• Coordinates: 2649229..2649591 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF696_RS13110
• Protein ID: WP_000948331.1
• Coordinates: 2649062..2649232 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF696_RS13080 (2644098)	2644098..2644658	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF696_RS13085 (2644867)	2644867..2645346	+	480	WP_001287079.1	hypothetical protein	-
IF696_RS13090 (2645339)	2645339..2646922	+	1584	WP_031864378.1	PH domain-containing protein	-
IF696_RS13095 (2646909)	2646909..2647400	+	492	WP_001205912.1	PH domain-containing protein	-
IF696_RS13100 (2647404)	2647404..2647763	+	360	WP_000581197.1	holo-ACP synthase	-
IF696_RS13105 (2647829)	2647829..2648977	+	1149	WP_001281154.1	alanine racemase	-
IF696_RS13110 (2649062)	2649062..2649232	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF696_RS13115 (2649229)	2649229..2649591	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF696_RS13120 (2649941)	2649941..2650942	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF696_RS13125 (2651061)	2651061..2651387	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF696_RS13130 (2651389)	2651389..2651868	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF696_RS13135 (2651843)	2651843..2652613	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175224 WP_000621175.1 NZ_CP062420:2649229-2649591 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175224 NZ_CP081824:c598-332 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175224 WP_000948331.1 NZ_CP062420:2649062-2649232 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175224 NZ_CP081824:c806-582 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1