Detailed information of TA system TA175218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 120069..120845
• Replicon: chromosome
• Accession: NZ_CP062420
• Organism: Staphylococcus aureus strain NAS_AN_016

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF696_RS00765
• Protein ID: WP_000031108.1
• Coordinates: 120693..120845 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: IF696_RS00760
• Protein ID: WP_001251224.1
• Coordinates: 120069..120668 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF696_RS00740 (115984)	115984..117441	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF696_RS00745 (117434)	117434..118156	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF696_RS00750 (118308)	118308..119435	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF696_RS00755 (119440)	119440..119910	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF696_RS00760 (120069)	120069..120668	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
IF696_RS00765 (120693)	120693..120845	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF696_RS00770 (121389)	121389..121784	+	396	WP_000901023.1	hypothetical protein	-
IF696_RS00775 (121980)	121980..123365	+	1386	WP_000116229.1	class II fumarate hydratase	-
IF696_RS00780 (123817)	123817..124638	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T175218 WP_000031108.1 NZ_CP062420:120693-120845 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T175218 NZ_CP081820:c4902261-4901977 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT175218 WP_001251224.1 NZ_CP062420:120069-120668 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT175218 NZ_CP081820:c4902493-4902251 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4