Detailed information of TA system TA175213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2646609..2647111
• Replicon: chromosome
• Accession: NZ_CP062419
• Organism: Staphylococcus aureus strain NAS_AN_023

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF697_RS13020
• Protein ID: WP_000581792.1
• Coordinates: 2646902..2647111 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: IF697_RS13015
• Protein ID: WP_240122451.1
• Coordinates: 2646609..2646890 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF697_RS12995 (2642213)	2642213..2642854	+	642	WP_000483153.1	thiamine phosphate synthase	-
IF697_RS13000 (2642940)	2642940..2643812	+	873	WP_000725802.1	membrane protein insertase YidC	-
IF697_RS13005 (2644284)	2644284..2644931	-	648	WP_001187630.1	HD domain-containing protein	-
IF697_RS13010 (2644959)	2644959..2646443	-	1485	WP_000571549.1	cardiolipin synthase	-
IF697_RS13015 (2646609)	2646609..2646890	+	282	WP_240122451.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF697_RS13020 (2646902)	2646902..2647111	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF697_RS13025 (2647239)	2647239..2647376	+	138	WP_000828354.1	Lmo0850 family protein	-
IF697_RS13030 (2647640)	2647640..2648842	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF697_RS13035 (2649160)	2649160..2650230	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF697_RS13040 (2650245)	2650245..2651603	+	1359	WP_000611470.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-87)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T175213 WP_000581792.1 NZ_CP062419:2646902-2647111 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T175213 NZ_CP081820:878303-878710 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10737.32 Da
• Isoelectric Point: 7.2447

>AT175213 WP_240122451.1 NZ_CP062419:2646609-2646890 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EELLVTFQKLIKD

Nucleotide

• Length: 282 bp

>AT175213 NZ_CP081820:878056-878322 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6FF1