Detailed information of TA system TA175195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2648965..2649494
• Replicon: chromosome
• Accession: NZ_CP062418
• Organism: Staphylococcus aureus strain NAS_AN_136

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF715_RS13095
• Protein ID: WP_000621175.1
• Coordinates: 2649132..2649494 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF715_RS13090
• Protein ID: WP_000948331.1
• Coordinates: 2648965..2649135 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF715_RS13060 (2644001)	2644001..2644561	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
IF715_RS13065 (2644770)	2644770..2645249	+	480	WP_001287079.1	hypothetical protein	-
IF715_RS13070 (2645242)	2645242..2646825	+	1584	WP_031864378.1	PH domain-containing protein	-
IF715_RS13075 (2646812)	2646812..2647303	+	492	WP_001205912.1	PH domain-containing protein	-
IF715_RS13080 (2647307)	2647307..2647666	+	360	WP_000581197.1	holo-ACP synthase	-
IF715_RS13085 (2647732)	2647732..2648880	+	1149	WP_001281154.1	alanine racemase	-
IF715_RS13090 (2648965)	2648965..2649135	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF715_RS13095 (2649132)	2649132..2649494	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF715_RS13100 (2649844)	2649844..2650845	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF715_RS13105 (2650964)	2650964..2651290	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF715_RS13110 (2651292)	2651292..2651771	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF715_RS13115 (2651746)	2651746..2652516	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175195 WP_000621175.1 NZ_CP062418:2649132-2649494 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175195 NZ_CP081813:c4201376-4201026 [Shigella flexneri]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175195 WP_000948331.1 NZ_CP062418:2648965-2649135 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175195 NZ_CP081813:c4201621-4201370 [Shigella flexneri]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1