Detailed information of TA system TA175194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2629226..2629740
• Replicon: chromosome
• Accession: NZ_CP062418
• Organism: Staphylococcus aureus strain NAS_AN_136

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: IF715_RS13005
• Protein ID: WP_000581792.1
• Coordinates: 2629531..2629740 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: IF715_RS13000
• Protein ID: WP_000138932.1
• Coordinates: 2629226..2629519 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF715_RS12980 (2624697)	2624697..2625338	+	642	WP_000483153.1	thiamine phosphate synthase	-
IF715_RS12985 (2625424)	2625424..2626296	+	873	WP_000725802.1	membrane protein insertase YidC	-
IF715_RS12990 (2626901)	2626901..2627548	-	648	WP_001187630.1	HD domain-containing protein	-
IF715_RS12995 (2627576)	2627576..2629060	-	1485	WP_000571549.1	cardiolipin synthase	-
IF715_RS13000 (2629226)	2629226..2629519	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IF715_RS13005 (2629531)	2629531..2629740	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
IF715_RS13010 (2629868)	2629868..2630005	+	138	WP_000828354.1	Lmo0850 family protein	-
IF715_RS13015 (2630269)	2630269..2631471	-	1203	WP_001836940.1	FtsW/RodA/SpoVE family cell cycle protein	-
IF715_RS13020 (2631789)	2631789..2632859	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
IF715_RS13025 (2632874)	2632874..2634232	+	1359	WP_000611471.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T175194 WP_000581792.1 NZ_CP062418:2629531-2629740 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T175194 NZ_CP081813:3753396-3753674 [Shigella flexneri]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT175194 WP_000138932.1 NZ_CP062418:2629226-2629519 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT175194 NZ_CP081813:3753133-3753393 [Shigella flexneri]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCATTTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13