Detailed information of TA system TA175184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2661380..2661909
• Replicon: chromosome
• Accession: NZ_CP062417
• Organism: Staphylococcus aureus strain NAS_AN_143

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF718_RS13085
• Protein ID: WP_000621175.1
• Coordinates: 2661547..2661909 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF718_RS13080
• Protein ID: WP_000948331.1
• Coordinates: 2661380..2661550 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF718_RS13050 (2656417)	2656417..2656977	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF718_RS13055 (2657185)	2657185..2657664	+	480	WP_240104172.1	hypothetical protein	-
IF718_RS13060 (2657657)	2657657..2659240	+	1584	WP_001294626.1	PH domain-containing protein	-
IF718_RS13065 (2659227)	2659227..2659718	+	492	WP_001205912.1	PH domain-containing protein	-
IF718_RS13070 (2659722)	2659722..2660081	+	360	WP_000581197.1	holo-ACP synthase	-
IF718_RS13075 (2660147)	2660147..2661295	+	1149	WP_001281154.1	alanine racemase	-
IF718_RS13080 (2661380)	2661380..2661550	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF718_RS13085 (2661547)	2661547..2661909	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF718_RS13090 (2662259)	2662259..2663260	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF718_RS13095 (2663379)	2663379..2663705	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF718_RS13100 (2663707)	2663707..2664186	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF718_RS13105 (2664161)	2664161..2664931	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175184 WP_000621175.1 NZ_CP062417:2661547-2661909 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175184 NZ_CP081813:1022827-1023162 [Shigella flexneri]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175184 WP_000948331.1 NZ_CP062417:2661380-2661550 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175184 NZ_CP081813:1022579-1022827 [Shigella flexneri]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1