Detailed information of TA system TA175167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2670087..2670616
• Replicon: chromosome
• Accession: NZ_CP062416
• Organism: Staphylococcus aureus strain NAS_AN_149

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF719_RS13115
• Protein ID: WP_000621175.1
• Coordinates: 2670254..2670616 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF719_RS13110
• Protein ID: WP_000948331.1
• Coordinates: 2670087..2670257 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF719_RS13080 (2665123)	2665123..2665683	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF719_RS13085 (2665892)	2665892..2666371	+	480	WP_001287090.1	hypothetical protein	-
IF719_RS13090 (2666364)	2666364..2667947	+	1584	WP_001294626.1	PH domain-containing protein	-
IF719_RS13095 (2667934)	2667934..2668425	+	492	WP_001205910.1	PH domain-containing protein	-
IF719_RS13100 (2668429)	2668429..2668788	+	360	WP_000581197.1	holo-ACP synthase	-
IF719_RS13105 (2668854)	2668854..2670002	+	1149	WP_001281142.1	alanine racemase	-
IF719_RS13110 (2670087)	2670087..2670257	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF719_RS13115 (2670254)	2670254..2670616	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF719_RS13120 (2670965)	2670965..2671966	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF719_RS13125 (2672085)	2672085..2672411	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF719_RS13130 (2672413)	2672413..2672892	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF719_RS13135 (2672867)	2672867..2673637	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175167 WP_000621175.1 NZ_CP062416:2670254-2670616 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175167 NZ_CP081791:836552-836836 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175167 WP_000948331.1 NZ_CP062416:2670087-2670257 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175167 NZ_CP081791:836317-836559 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1