Detailed information of TA system TA175154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 129156..129932
• Replicon: chromosome
• Accession: NZ_CP062416
• Organism: Staphylococcus aureus strain NAS_AN_149

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: IF719_RS00815
• Protein ID: WP_000031108.1
• Coordinates: 129780..129932 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: IF719_RS00810
• Protein ID: WP_001251229.1
• Coordinates: 129156..129755 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF719_RS00790 (125073)	125073..126530	+	1458	WP_000649907.1	ABC transporter permease subunit	-
IF719_RS00795 (126523)	126523..127245	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
IF719_RS00800 (127396)	127396..128523	+	1128	WP_029160281.1	tRNA epoxyqueuosine(34) reductase QueG	-
IF719_RS00805 (128528)	128528..128998	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
IF719_RS00810 (129156)	129156..129755	+	600	WP_001251229.1	glucosamine-6-phosphate isomerase	Antitoxin
IF719_RS00815 (129780)	129780..129932	+	153	WP_000031108.1	SAS053 family protein	Toxin
IF719_RS00820 (130766)	130766..131161	+	396	WP_000901023.1	hypothetical protein	-
IF719_RS00825 (131357)	131357..132742	+	1386	WP_000116231.1	class II fumarate hydratase	-
IF719_RS00830 (133195)	133195..134016	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T175154 WP_000031108.1 NZ_CP062416:129780-129932 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T175154 NZ_CP081783:54145-54429 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22329.45 Da
• Isoelectric Point: 5.1445

>AT175154 WP_001251229.1 NZ_CP062416:129156-129755 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEGLGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT175154 NZ_CP081783:53910-54152 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Toxin

• AlphaFold DB: X5E2E6