175149

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2813957..2814486 Replicon chromosome
Accession NZ_CP062415
Organism Staphylococcus aureus strain NAS_AN_150

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag IF720_RS13980 Protein ID WP_000621176.1
Coordinates 2814124..2814486 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag IF720_RS13975 Protein ID WP_000948331.1
Coordinates 2813957..2814127 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF720_RS13945 (2808993) 2808993..2809553 + 561 WP_001092414.1 K(+)-transporting ATPase subunit C -
IF720_RS13950 (2809762) 2809762..2810241 + 480 WP_001287082.1 hypothetical protein -
IF720_RS13955 (2810234) 2810234..2811811 + 1578 WP_001294646.1 PH domain-containing protein -
IF720_RS13960 (2811804) 2811804..2812295 + 492 WP_001286801.1 PH domain-containing protein -
IF720_RS13965 (2812299) 2812299..2812658 + 360 WP_000581197.1 holo-ACP synthase -
IF720_RS13970 (2812724) 2812724..2813872 + 1149 WP_001281140.1 alanine racemase -
IF720_RS13975 (2813957) 2813957..2814127 + 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IF720_RS13980 (2814124) 2814124..2814486 + 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IF720_RS13985 (2814835) 2814835..2815836 + 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
IF720_RS13990 (2815955) 2815955..2816281 + 327 WP_216501389.1 anti-sigma factor antagonist -
IF720_RS13995 (2816283) 2816283..2816762 + 480 WP_001190825.1 anti-sigma B factor RsbW -
IF720_RS14000 (2816737) 2816737..2817507 + 771 WP_001041107.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T175149 WP_000621176.1 NZ_CP062415:2814124-2814486 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T175149 NZ_CP081782:c4151448-4151134 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT175149 WP_000948331.1 NZ_CP062415:2813957-2814127 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT175149 NZ_CP081782:c4151684-4151451 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References