Detailed information of TA system TA175140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1289434..1289911
• Replicon: chromosome
• Accession: NZ_CP062415
• Organism: Staphylococcus aureus strain NAS_AN_150

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: IF720_RS06590
• Protein ID: WP_000052076.1
• Coordinates: 1289723..1289911 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: IF720_RS06585
• Protein ID: WP_001059690.1
• Coordinates: 1289434..1289694 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF720_RS06550 (1284826)	1284826..1285542	-	717	WP_000150036.1	type I 3-dehydroquinate dehydratase	-
IF720_RS06555 (1285690)	1285690..1286112	+	423	WP_000974455.1	organic hydroperoxide resistance protein	-
IF720_RS06560 (1286511)	1286511..1287005	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
IF720_RS06565 (1287161)	1287161..1287778	+	618	WP_000255545.1	LysE/ArgO family amino acid transporter	-
IF720_RS06570 (1287851)	1287851..1288432	-	582	WP_001140696.1	histidine phosphatase family protein	-
IF720_RS06575 (1288657)	1288657..1288893	+	237	WP_000368687.1	hypothetical protein	-
IF720_RS06580 (1288890)	1288890..1289093	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
IF720_RS06585 (1289434)	1289434..1289694	+	261	WP_001059690.1	hypothetical protein	Antitoxin
IF720_RS06590 (1289723)	1289723..1289911	+	189	WP_000052076.1	hypothetical protein	Toxin
IF720_RS06595 (1290099)	1290099..1290668	+	570	WP_001106663.1	lipoprotein N-acylation protein LnsA	-
IF720_RS06600 (1290756)	1290756..1291040	+	285	WP_001004401.1	hypothetical protein	-
IF720_RS06605 (1291102)	1291102..1291320	+	219	WP_000005879.1	hypothetical protein	-
IF720_RS06610 (1291817)	1291817..1292017	-	201	WP_001059082.1	cold-shock protein	-
IF720_RS06615 (1292374)	1292374..1293060	-	687	WP_001574556.1	thermonuclease family protein	-
IF720_RS06620 (1293401)	1293401..1293958	-	558	WP_000824491.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T175140 WP_000052076.1 NZ_CP062415:1289723-1289911 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T175140 NZ_CP081782:1969849-1970100 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT175140 WP_001059690.1 NZ_CP062415:1289434-1289694 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT175140 NZ_CP081782:1970097-1970351 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56