Detailed information of TA system TA175130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2670753..2671282
• Replicon: chromosome
• Accession: NZ_CP062414
• Organism: Staphylococcus aureus strain NAS_AN_175

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF723_RS13130
• Protein ID: WP_000621175.1
• Coordinates: 2670920..2671282 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF723_RS13125
• Protein ID: WP_000948331.1
• Coordinates: 2670753..2670923 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF723_RS13095 (2665789)	2665789..2666349	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
IF723_RS13100 (2666558)	2666558..2667037	+	480	WP_001287090.1	hypothetical protein	-
IF723_RS13105 (2667030)	2667030..2668613	+	1584	WP_001294626.1	PH domain-containing protein	-
IF723_RS13110 (2668600)	2668600..2669091	+	492	WP_001205910.1	PH domain-containing protein	-
IF723_RS13115 (2669095)	2669095..2669454	+	360	WP_000581197.1	holo-ACP synthase	-
IF723_RS13120 (2669520)	2669520..2670668	+	1149	WP_001281142.1	alanine racemase	-
IF723_RS13125 (2670753)	2670753..2670923	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF723_RS13130 (2670920)	2670920..2671282	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF723_RS13135 (2671631)	2671631..2672632	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF723_RS13140 (2672751)	2672751..2673077	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF723_RS13145 (2673079)	2673079..2673558	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF723_RS13150 (2673533)	2673533..2674303	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175130 WP_000621175.1 NZ_CP062414:2670920-2671282 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175130 NZ_CP081775:261162-261578 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175130 WP_000948331.1 NZ_CP062414:2670753-2670923 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175130 NZ_CP081775:260935-261165 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1