Detailed information of TA system TA175112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2704525..2705054
• Replicon: chromosome
• Accession: NZ_CP062413
• Organism: Staphylococcus aureus strain NAS_AN_176

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IF724_RS13400
• Protein ID: WP_000621175.1
• Coordinates: 2704692..2705054 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IF724_RS13395
• Protein ID: WP_000948331.1
• Coordinates: 2704525..2704695 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF724_RS13365 (2699562)	2699562..2700122	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
IF724_RS13370 (2700330)	2700330..2700809	+	480	WP_240104172.1	hypothetical protein	-
IF724_RS13375 (2700802)	2700802..2702385	+	1584	WP_001294626.1	PH domain-containing protein	-
IF724_RS13380 (2702372)	2702372..2702863	+	492	WP_001205912.1	PH domain-containing protein	-
IF724_RS13385 (2702867)	2702867..2703226	+	360	WP_000581197.1	holo-ACP synthase	-
IF724_RS13390 (2703292)	2703292..2704440	+	1149	WP_001281154.1	alanine racemase	-
IF724_RS13395 (2704525)	2704525..2704695	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IF724_RS13400 (2704692)	2704692..2705054	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IF724_RS13405 (2705404)	2705404..2706405	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IF724_RS13410 (2706524)	2706524..2706850	+	327	WP_001052491.1	anti-sigma factor antagonist	-
IF724_RS13415 (2706852)	2706852..2707331	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
IF724_RS13420 (2707306)	2707306..2708076	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T175112 WP_000621175.1 NZ_CP062413:2704692-2705054 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T175112 NZ_CP081774:1046791-1047126 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAAGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT175112 WP_000948331.1 NZ_CP062413:2704525-2704695 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT175112 NZ_CP081774:1046543-1046791 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1