Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2306573..2307096 Replicon chromosome
Accession NZ_CP062413
Organism Staphylococcus aureus strain NAS_AN_176

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag IF724_RS11345 Protein ID WP_000074049.1
Coordinates 2306830..2307096 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag IF724_RS11340 Protein ID WP_000587616.1
Coordinates 2306573..2306830 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF724_RS11325 (2303071) 2303071..2303820 - 750 WP_000072461.1 CPBP family lipoprotein N-acylation protein LnsB -
IF724_RS11330 (2304015) 2304015..2305592 + 1578 WP_000143494.1 FMN-binding glutamate synthase family protein -
IF724_RS11335 (2305760) 2305760..2306215 - 456 WP_000732076.1 DUF1307 domain-containing protein -
IF724_RS11340 (2306573) 2306573..2306830 + 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IF724_RS11345 (2306830) 2306830..2307096 + 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
IF724_RS11350 (2307451) 2307451..2308527 + 1077 WP_001076673.1 M42 family metallopeptidase -
IF724_RS11355 (2308775) 2308775..2309437 + 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
IF724_RS11360 (2309430) 2309430..2310206 + 777 WP_000072149.1 iron export ABC transporter permease subunit FetB -
IF724_RS11365 (2310602) 2310602..2311789 + 1188 WP_000026189.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T175103 WP_000074049.1 NZ_CP062413:2306830-2307096 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T175103 NZ_CP081768:291552-291836 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT175103 WP_000587616.1 NZ_CP062413:2306573-2306830 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT175103 NZ_CP081768:291317-291559 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References